aav2 aav hsyn dio egfp addgene Search Results


aav2 e f1a  (Addgene inc)
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vector aav2 1 hsynapsin gcamp6s addgene  (Addgene inc)
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plasmid pucmini icap php eb encoding modified aav2 9 capsid  (Addgene inc)
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aav2 1 ef1á dio hchr2 h134r  (Addgene inc)
96
Addgene inc aav2 1 ef1á dio hchr2 h134r
Summary of the viral constructs used DOI: http://dx.doi.org/10.7554/eLife.06262.012
Aav2 1 Ef1á Dio Hchr2 H134r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2 backbone plasmid  (Addgene inc)
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Summary of the viral constructs used DOI: http://dx.doi.org/10.7554/eLife.06262.012
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fluorophore mcherry aav2 hsyn diohm3d gq mcherry  (Addgene inc)
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Summary of the viral constructs used DOI: http://dx.doi.org/10.7554/eLife.06262.012
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virus strains aav2 5 cag  (Jackson Immuno)
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Jackson Immuno virus strains aav2 5 cag
(A) Schematic of VTA-DA and SNc-DA targeting strategy using TH::Cre rats and Cre-dependent <t>AAV2/5</t> DIO-NpHR-YFP (or DIO-YFP for the control group) virus injected into the VTA (top) or SNc (bottom), respectively.
Virus Strains Aav2 5 Cag, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retro aav2 ef1a nuc flox mcherry egfp  (Addgene inc)
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Retro Aav2 Ef1a Nuc Flox Mcherry Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2 ewb dio myrtagrfp tp2a post egrasp  (Addgene inc)
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Image Search Results


Summary of the viral constructs used DOI: http://dx.doi.org/10.7554/eLife.06262.012

Journal: eLife

Article Title: A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity

doi: 10.7554/eLife.06262

Figure Lengend Snippet: Summary of the viral constructs used DOI: http://dx.doi.org/10.7554/eLife.06262.012

Article Snippet: AAV2.1.EF1á.DIO.hChR2(H134R).eYFP , Addgene 20,298 , GlyT2-cre (CN) , 1B, F, 2B, D, 3B–D, 6A, C.

Techniques: Construct, Injection

(A) Schematic of VTA-DA and SNc-DA targeting strategy using TH::Cre rats and Cre-dependent AAV2/5 DIO-NpHR-YFP (or DIO-YFP for the control group) virus injected into the VTA (top) or SNc (bottom), respectively.

Journal: Cell reports

Article Title: A Comparison of Dopaminergic and Cholinergic Populations Reveals Unique Contributions of VTA Dopamine Neurons to Short-Term Memory

doi: 10.1016/j.celrep.2020.108492

Figure Lengend Snippet: (A) Schematic of VTA-DA and SNc-DA targeting strategy using TH::Cre rats and Cre-dependent AAV2/5 DIO-NpHR-YFP (or DIO-YFP for the control group) virus injected into the VTA (top) or SNc (bottom), respectively.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-TH Aves lab Cat# TYH; RRID: AB_10013440 Goat monoclonal anti-ChAT Millipore Cat# AB144P; RRID: AB_2079751 Rabbit monoclonal anti-GFP Thermo Fisher Scientific Cat# G10362 ; RRID: AB_2536526 Donkey anti-Chicken conjugated to Cy3 Jackson Immuno Research Cat# 703-165-155; RRID: AB_2340363 Donkey anti-Goat conjugated to Alexa Fluor 647 Jackson Immuno Research Cat# 705-605-147; RRID: AB_2340437 Donkey anti-Rabbit conjugated to Alexa Fluor 488 Jackson Immuno Research Cat# 711-545-152; RRID: AB_2313584 Bacterial and Virus Strains AAV2/5-CAG-Flex-GCamP6f U Penn Vector Core https://www.addgene.org/100835/ ; RRID: Addgene_100835 AAV2/5-CAG-DIO-RatOpt-GCaMP6f PNI Viral Core, Princeton Cat# AAV-VC58 AAV2/5-CAG-Flex-eGFP U Penn Vector Core https://www.addgene.org/51502/ ; RRID: Addgene_51502 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP U Penn Vector Core https://www.addgene.org/26966/ ; RRID: Addgene_26966 AAV2/5-EF1a-DIO-ChR2-eYFP U Penn Vector Core https://www.addgene.org/20298/ ; RRID: Addgene_20298 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP PNI Viral Core, Princeton Cat# AAV-VC24 AAV2/5-EF1a-DIO-ChR2-eYFP PNI Viral Core, Princeton Cat# AAV-VC53 AAV2/5-EF1a-DIO-eYFP PNI Viral Core, Princeton Cat# AAV-VC93 AAV2/5-CMV-DIO-TVA66T-HA-P2A-N2cΔG PNI Viral Core, Princeton Cat# AAV-VC178 RabV-CVS-N2cΔG-mCherry/EnvA Obtained by PNI Viral Core from The Center for Neuroanatomy with Neurotropic Viruses (CNNV) https://www.addgene.org/73464/ ; RRID: Addgene_73464 Experimental Models: Organisms/Strains Rat: TH::Cre Long Evans Horizon Cat# TGRA8400 Rat: ChAT::Cre Long Evans RRRC Cat# 658 Software and Algorithms U test stata module Lind and Mehlum, 2010 https://econpapers.repec.org/software/bocbocode/s456874.htm Wholebrain R package Fürth et al., 2018 http://www.wholebrainsoftware.org/ Other Fibers for optogenetics Thor labs Cat# FT300UMT Ferrules for optogenetics Precision Fiber Products Cat# MM-FER-2006SS-3300 Patch cord for fiberphotometry Doric Lenses Cat# MFP_400/430/1100-0.57_0.45m_FCM-MF2.5_LAF Ferrules for fiberphotometry Doric Lenses Cat# MFC_400/430-0.48_10mm_MF2.5_FLT Open in a separate window KEY RESOURCES TABLE Recording and manipulation of four neuromodulatory populations during short-term memory Only dopamine neurons in VTA contribute selectively to short-term memory Contribution is during the delay period, despite low activity at that time Inverted-U relationship between VTA dopamine activity and performance

Techniques: Injection

(A) Schematic of NB-ChAT and MS-ChAT targeting strategy using ChAT::Cre rats and AAV2/5 DIO-NpHR-YFP virus injected into the NB (top) or MS (bottom).

Journal: Cell reports

Article Title: A Comparison of Dopaminergic and Cholinergic Populations Reveals Unique Contributions of VTA Dopamine Neurons to Short-Term Memory

doi: 10.1016/j.celrep.2020.108492

Figure Lengend Snippet: (A) Schematic of NB-ChAT and MS-ChAT targeting strategy using ChAT::Cre rats and AAV2/5 DIO-NpHR-YFP virus injected into the NB (top) or MS (bottom).

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-TH Aves lab Cat# TYH; RRID: AB_10013440 Goat monoclonal anti-ChAT Millipore Cat# AB144P; RRID: AB_2079751 Rabbit monoclonal anti-GFP Thermo Fisher Scientific Cat# G10362 ; RRID: AB_2536526 Donkey anti-Chicken conjugated to Cy3 Jackson Immuno Research Cat# 703-165-155; RRID: AB_2340363 Donkey anti-Goat conjugated to Alexa Fluor 647 Jackson Immuno Research Cat# 705-605-147; RRID: AB_2340437 Donkey anti-Rabbit conjugated to Alexa Fluor 488 Jackson Immuno Research Cat# 711-545-152; RRID: AB_2313584 Bacterial and Virus Strains AAV2/5-CAG-Flex-GCamP6f U Penn Vector Core https://www.addgene.org/100835/ ; RRID: Addgene_100835 AAV2/5-CAG-DIO-RatOpt-GCaMP6f PNI Viral Core, Princeton Cat# AAV-VC58 AAV2/5-CAG-Flex-eGFP U Penn Vector Core https://www.addgene.org/51502/ ; RRID: Addgene_51502 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP U Penn Vector Core https://www.addgene.org/26966/ ; RRID: Addgene_26966 AAV2/5-EF1a-DIO-ChR2-eYFP U Penn Vector Core https://www.addgene.org/20298/ ; RRID: Addgene_20298 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP PNI Viral Core, Princeton Cat# AAV-VC24 AAV2/5-EF1a-DIO-ChR2-eYFP PNI Viral Core, Princeton Cat# AAV-VC53 AAV2/5-EF1a-DIO-eYFP PNI Viral Core, Princeton Cat# AAV-VC93 AAV2/5-CMV-DIO-TVA66T-HA-P2A-N2cΔG PNI Viral Core, Princeton Cat# AAV-VC178 RabV-CVS-N2cΔG-mCherry/EnvA Obtained by PNI Viral Core from The Center for Neuroanatomy with Neurotropic Viruses (CNNV) https://www.addgene.org/73464/ ; RRID: Addgene_73464 Experimental Models: Organisms/Strains Rat: TH::Cre Long Evans Horizon Cat# TGRA8400 Rat: ChAT::Cre Long Evans RRRC Cat# 658 Software and Algorithms U test stata module Lind and Mehlum, 2010 https://econpapers.repec.org/software/bocbocode/s456874.htm Wholebrain R package Fürth et al., 2018 http://www.wholebrainsoftware.org/ Other Fibers for optogenetics Thor labs Cat# FT300UMT Ferrules for optogenetics Precision Fiber Products Cat# MM-FER-2006SS-3300 Patch cord for fiberphotometry Doric Lenses Cat# MFP_400/430/1100-0.57_0.45m_FCM-MF2.5_LAF Ferrules for fiberphotometry Doric Lenses Cat# MFC_400/430-0.48_10mm_MF2.5_FLT Open in a separate window KEY RESOURCES TABLE Recording and manipulation of four neuromodulatory populations during short-term memory Only dopamine neurons in VTA contribute selectively to short-term memory Contribution is during the delay period, despite low activity at that time Inverted-U relationship between VTA dopamine activity and performance

Techniques: Injection

(A) Schematic of VTA-DA targeting strategy using TH::Cre rats and AAV2/5 DIO-ChR2-YFP virus.

Journal: Cell reports

Article Title: A Comparison of Dopaminergic and Cholinergic Populations Reveals Unique Contributions of VTA Dopamine Neurons to Short-Term Memory

doi: 10.1016/j.celrep.2020.108492

Figure Lengend Snippet: (A) Schematic of VTA-DA targeting strategy using TH::Cre rats and AAV2/5 DIO-ChR2-YFP virus.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-TH Aves lab Cat# TYH; RRID: AB_10013440 Goat monoclonal anti-ChAT Millipore Cat# AB144P; RRID: AB_2079751 Rabbit monoclonal anti-GFP Thermo Fisher Scientific Cat# G10362 ; RRID: AB_2536526 Donkey anti-Chicken conjugated to Cy3 Jackson Immuno Research Cat# 703-165-155; RRID: AB_2340363 Donkey anti-Goat conjugated to Alexa Fluor 647 Jackson Immuno Research Cat# 705-605-147; RRID: AB_2340437 Donkey anti-Rabbit conjugated to Alexa Fluor 488 Jackson Immuno Research Cat# 711-545-152; RRID: AB_2313584 Bacterial and Virus Strains AAV2/5-CAG-Flex-GCamP6f U Penn Vector Core https://www.addgene.org/100835/ ; RRID: Addgene_100835 AAV2/5-CAG-DIO-RatOpt-GCaMP6f PNI Viral Core, Princeton Cat# AAV-VC58 AAV2/5-CAG-Flex-eGFP U Penn Vector Core https://www.addgene.org/51502/ ; RRID: Addgene_51502 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP U Penn Vector Core https://www.addgene.org/26966/ ; RRID: Addgene_26966 AAV2/5-EF1a-DIO-ChR2-eYFP U Penn Vector Core https://www.addgene.org/20298/ ; RRID: Addgene_20298 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP PNI Viral Core, Princeton Cat# AAV-VC24 AAV2/5-EF1a-DIO-ChR2-eYFP PNI Viral Core, Princeton Cat# AAV-VC53 AAV2/5-EF1a-DIO-eYFP PNI Viral Core, Princeton Cat# AAV-VC93 AAV2/5-CMV-DIO-TVA66T-HA-P2A-N2cΔG PNI Viral Core, Princeton Cat# AAV-VC178 RabV-CVS-N2cΔG-mCherry/EnvA Obtained by PNI Viral Core from The Center for Neuroanatomy with Neurotropic Viruses (CNNV) https://www.addgene.org/73464/ ; RRID: Addgene_73464 Experimental Models: Organisms/Strains Rat: TH::Cre Long Evans Horizon Cat# TGRA8400 Rat: ChAT::Cre Long Evans RRRC Cat# 658 Software and Algorithms U test stata module Lind and Mehlum, 2010 https://econpapers.repec.org/software/bocbocode/s456874.htm Wholebrain R package Fürth et al., 2018 http://www.wholebrainsoftware.org/ Other Fibers for optogenetics Thor labs Cat# FT300UMT Ferrules for optogenetics Precision Fiber Products Cat# MM-FER-2006SS-3300 Patch cord for fiberphotometry Doric Lenses Cat# MFP_400/430/1100-0.57_0.45m_FCM-MF2.5_LAF Ferrules for fiberphotometry Doric Lenses Cat# MFC_400/430-0.48_10mm_MF2.5_FLT Open in a separate window KEY RESOURCES TABLE Recording and manipulation of four neuromodulatory populations during short-term memory Only dopamine neurons in VTA contribute selectively to short-term memory Contribution is during the delay period, despite low activity at that time Inverted-U relationship between VTA dopamine activity and performance

Techniques:

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: A Comparison of Dopaminergic and Cholinergic Populations Reveals Unique Contributions of VTA Dopamine Neurons to Short-Term Memory

doi: 10.1016/j.celrep.2020.108492

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-TH Aves lab Cat# TYH; RRID: AB_10013440 Goat monoclonal anti-ChAT Millipore Cat# AB144P; RRID: AB_2079751 Rabbit monoclonal anti-GFP Thermo Fisher Scientific Cat# G10362 ; RRID: AB_2536526 Donkey anti-Chicken conjugated to Cy3 Jackson Immuno Research Cat# 703-165-155; RRID: AB_2340363 Donkey anti-Goat conjugated to Alexa Fluor 647 Jackson Immuno Research Cat# 705-605-147; RRID: AB_2340437 Donkey anti-Rabbit conjugated to Alexa Fluor 488 Jackson Immuno Research Cat# 711-545-152; RRID: AB_2313584 Bacterial and Virus Strains AAV2/5-CAG-Flex-GCamP6f U Penn Vector Core https://www.addgene.org/100835/ ; RRID: Addgene_100835 AAV2/5-CAG-DIO-RatOpt-GCaMP6f PNI Viral Core, Princeton Cat# AAV-VC58 AAV2/5-CAG-Flex-eGFP U Penn Vector Core https://www.addgene.org/51502/ ; RRID: Addgene_51502 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP U Penn Vector Core https://www.addgene.org/26966/ ; RRID: Addgene_26966 AAV2/5-EF1a-DIO-ChR2-eYFP U Penn Vector Core https://www.addgene.org/20298/ ; RRID: Addgene_20298 AAV2/5-EF1a-DIO-eNpHR3.0-eYFP PNI Viral Core, Princeton Cat# AAV-VC24 AAV2/5-EF1a-DIO-ChR2-eYFP PNI Viral Core, Princeton Cat# AAV-VC53 AAV2/5-EF1a-DIO-eYFP PNI Viral Core, Princeton Cat# AAV-VC93 AAV2/5-CMV-DIO-TVA66T-HA-P2A-N2cΔG PNI Viral Core, Princeton Cat# AAV-VC178 RabV-CVS-N2cΔG-mCherry/EnvA Obtained by PNI Viral Core from The Center for Neuroanatomy with Neurotropic Viruses (CNNV) https://www.addgene.org/73464/ ; RRID: Addgene_73464 Experimental Models: Organisms/Strains Rat: TH::Cre Long Evans Horizon Cat# TGRA8400 Rat: ChAT::Cre Long Evans RRRC Cat# 658 Software and Algorithms U test stata module Lind and Mehlum, 2010 https://econpapers.repec.org/software/bocbocode/s456874.htm Wholebrain R package Fürth et al., 2018 http://www.wholebrainsoftware.org/ Other Fibers for optogenetics Thor labs Cat# FT300UMT Ferrules for optogenetics Precision Fiber Products Cat# MM-FER-2006SS-3300 Patch cord for fiberphotometry Doric Lenses Cat# MFP_400/430/1100-0.57_0.45m_FCM-MF2.5_LAF Ferrules for fiberphotometry Doric Lenses Cat# MFC_400/430-0.48_10mm_MF2.5_FLT Open in a separate window KEY RESOURCES TABLE Recording and manipulation of four neuromodulatory populations during short-term memory Only dopamine neurons in VTA contribute selectively to short-term memory Contribution is during the delay period, despite low activity at that time Inverted-U relationship between VTA dopamine activity and performance

Techniques: Plasmid Preparation, Software, Optogenetics

Journal: eLife

Article Title: Glutamatergic supramammillary nucleus neurons respond to threatening stressors and promote active coping

doi: 10.7554/eLife.90972

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Retro-AAV2-EF1a-Nuc-flox(mCherry)-EGFP (7×1012 vg/mL) , Addgene , RRID: Addgene_ 112677-AAVrg , .

Techniques: Recombinant, Plasmid Preparation, Software, Fluorescence, Microscopy

Journal: eLife

Article Title: Glutamatergic supramammillary nucleus neurons respond to threatening stressors and promote active coping

doi: 10.7554/eLife.90972

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Retro-AAV2-EF1a-Nuc-flox(mCherry)-EGFP (7×1012 vg/mL) , Addgene , RRID: Addgene_ 112677-AAVrg , .

Techniques: Virus